Frequently Asked Questions
MMC is not a stand-alone Covid-19 test. Instead, it is used to concentrate Covid-19 antigens in mouth wash and gargle samples to be used in further tests like PCR or rapid antigen tests.
We recommend using water with low ionic strength or best deionized water if available.
Do not use mineral water and saline solutions as they interfere with the PCR or rapid antigen tests.
The person taking the test should first gargle and rinse his/her mouth with gargling water rigorously for at least 15 seconds and spit it into the MMC cup.
Our experience suggests that children older than 5 years of age can easily gargle and rinse their mouth with water. Children younger than 5 years of age may give sample only as mouth wash.
Waiting more than 5 minutes may cause dried out samples. If it is the case and if it is not possible to pull out any
sample, add 500μl of the original sample or water, mix and pull out the concentrated sample. Your sample will still be concentrated. If MyMagiCon powder is in filter bag, the bag will limit the amount of absorbed sample and there will be no drying problem.
If more than 20 ml sample is used, then the concentration factor will be reduced, however, the sample will still be concentrated according to the volume used.
Using less than 20 ml sample can result in the formation of dried out sample. If it is the case and if it is not possible to pull out any sample, add 500μl of the original sample or water, mix and pull out the concentrated sample. Your sample will still be concentrated.
MMC contains a special polymer that removes small molecules quickly from solutions and concentrates microorganisms and macromolecules. MyMagiCon–RW100®, which is intended to be used for concentrating gargle and mouth wash, for the diagnosis of infectious agents like SARS-CoV2, Influenza virus and other viruses causing infection in the respiratory system, concentrates the sample 10 to 20 times in only 5 minutes.
This technique has several advantages:
a. It eliminates the need to obtain nasopharyngeal sample, which is invasive and poses a risk of infection for the healthcare professional who collects the sample.
b. Only about 50µl liquid can be collected by nasopharyngeal sampling, which is then diluted about 60 times, which in turn leads to reduced sensitivity for the further tests.
c. Clinical study RT-PCR results indicated that concentrated gargle and mouthwash sample can be used instead of nasopharyngeal sample.
d. This technique allows easy sampling and increases the sensitivity of further tests.
e. People will be able to collect their own samples and either test themselves at home or safely transfer their sample for laboratory testing.
We have tested the stability of viral RNA in virus spiked mouthwash samples from healthy volunteers. There was no significant change in the copy number of SARS-CoV-2 RNA after three days of storage at both room temperature and 4°C. There was about one log decrease after storing 10 days at both of these temperatures. Storing mouthwash samples either at room temperature or at 4°C, showed no significant difference in the stability of viral RNA.
In a cohort of 363 clinical samples, 101 gargle and mouth wash samples concentrated with MyMagiCon was PCR positive compared to 76 nasopharyngeal swap PCR positive samples. This brings about 66.7% positives in nasopharyngeal swap and 88.6% MyMagiCon concentrated mouth wash samples.
Concentrated sample can be tested using any of the available Covid-19 diagnosis procedure like PCR or rapid antigen testing.
MyMagiCon–RW100®, which is intended to be used for concentrating gargle and mouth wash, for the diagnosis of infectious agents like SARS-CoV2, Influenza virus and other viruses causing infection in the respiratory system.
It has been shown that SARS-CoV2 virus is well protected in gargle and mouthwash for 3 days at room temperature before analysis.
Gargle and mouthwash samples can be kept in the MyMagiCon tubes until analysis.